Induction of inflammation by Escherichia coli on the mucosal level: requirement for adherence and endotoxin.

Bacterial infection of the mouse urinary tract is followed by the recruitment of leukocytes to the mucosal surface. This study examined the bacterial components involved in the induction of this response. Escherichia coli of serotype O75:K5:H- expressing adhesins specific for the Gal alpha 1-4Gal beta- (Gal, galactose) and mannose-containing receptors were instilled into the urinary bladder of lipopolysaccharide responder (C3H/HcN) and lipopolysaccharide nonresponder (C3H/HeJ) mice.

An experimental model for ascending acute pyelonephritis caused by Escherichia coli or proteus in rats.

Experimental, ascending acute pyelonephritis in rats was produced by injecting 0 x 5 ml of 10(9) bacteria/ml into the urinary bladder via the urethra. No traumatic manipulation of the ureters of kidneys was necessary. A grading system for kidney lesions based on macro- and microscopical examination was used.

Protective effect of immunization with Salmonella minnesota Re 595 against ascending Escherichia coli O6K13H1 pyelonephritis in rats.

Active as well as passive immunization with formalin-killed S. minnesota Re 595 bacteria protected against experimental, ascending pyelonephritis caused by E. coli O6K13H1 in rats.