A Hot-Swappable Genetic Switch: Building an inducible and trackable functional assay for the essential gene MEDIATOR 21.

Essential genes, estimated at approximately 20% of the genome, are broadly expressed and required for reproductive success. They are difficult to study, as interfering with their function leads to premature death. Transcription is one of the essential functions of life, and the multi-protein Mediator complex coordinates the regulation of gene expression at nearly every eukaryotic promoter.

A function of TPL/TBL1-type corepressors is to nucleate the assembly of the preinitiation complex.

The plant corepressor TPL is recruited to diverse chromatin contexts, yet its mechanism of repression remains unclear. Previously, we leveraged the fact that TPL retains its function in a synthetic transcriptional circuit in the yeast model Saccharomyces cerevisiae to localize repressive function to two distinct domains. Here, we employed two unbiased whole-genome approaches to map the physical and genetic interactions of TPL at a repressed locus.

Co-pyrolysis of corn stalk and high-density polyethylene with emphasis on the fibrous tissue difference on thermal behavior and kinetics.

The thermal properties of various corn stalk tissues (including stem, husk, ear, cob, and leaf), high-density polyethylene (HDPE), and their blends were investigated using thermogravimetric analysis under a nitrogen atmosphere. The results indicate that the thermal decomposition process of corn stalk tissue/HDPE mixtures is delayed with an increasing heating rate, regardless of the tissue type. Besides, the structural differences among various corn stalk tissues significantly influence their thermal behavior, product distribution, and co-pyrolysis kinetics with HDPE.

Search for Soft Unclustered Energy Patterns in Proton-Proton Collisions at 13 TeV.

The first search for soft unclustered energy patterns (SUEPs) is performed using an integrated luminosity of 138  fb^{-1} of proton-proton collision data at sqrt[s]=13  TeV, collected in 2016-2018 by the CMS detector at the LHC. Such SUEPs are predicted by hidden valley models with a new, confining force with a large 't Hooft coupling. In events with boosted topologies, selected by high-threshold hadronic triggers, the multiplicity and sphericity of clustered tracks are used to reject the background from standard model quantum chromodynamics.