Publications by authors named "I M Van Kampen"
Article Synopsis
- Filamentous microorganisms, like fungi, have complex structures made up of branched hyphae, and accurately measuring the mechanical properties of these hyphae is essential for understanding their behavior.
- This study introduces a novel microfluidic system that measures the bending stiffness of individual fungal hyphae more accurately by positioning the samples away from interfering walls, resulting in better measurement outcomes.
- The findings indicate that the bending stiffness of certain hyphae is significantly higher than previously recorded, while the Young's Modulus, describing the cell wall elasticity, shows similar values across different fungal types, aiding future research on how cultivation conditions affect hyphal properties and overall fungus behavior.
Article Synopsis
- The survival of probiotic microorganisms like Saccharomyces cerevisiae is crucial for their health benefits, necessitating careful handling during production, particularly in drying and tableting processes.* -
- Previous research identified protective additives for spray-drying and explored the effects of tablet formulation, but there was little focus on the impact of multiple compressions during the tableting process.* -
- The study found that compressing tablets up to five times does not significantly change tablet strength or microorganism survival, suggesting that earlier findings from single compression studies are applicable, and that the inactivation of probiotics during tableting is linked to porosity reduction rather than compression methods.*
Future industrial applications of microparticle fractionation with deterministic lateral displacement (DLD) devices are hindered by exceedingly low throughput rates. To enable the necessary high-volume flows, high flow velocities as well as high aspect ratios in DLD devices have to be investigated. However, no experimental studies have yet been conducted on the fractionation of bi-disperse suspensions containing particles below 10 µm with DLD at a Reynolds number (Re) above 60.
View Article and Find Full Text PDFIn biotechnological processes, filamentous microorganisms are known for their broad product spectrum and complex cellular morphology. Product formation and cellular morphology are often closely linked, requiring a well-defined level of mechanical stress to achieve high product concentrations. Macroparticles were added to shake flask cultures of the filamentous actinomycete to find these optimal cultivation conditions.
View Article and Find Full Text PDFTemperature influence on the compression and breakage behaviour of yeast cells.
Lett Appl Microbiol
August 2023
Article Synopsis
- Industrial biotechnology utilizes microbial cells, like yeast, to create various products, but their mechanical properties are less understood compared to their genetic properties.
- A test procedure using nanoindentation measures the compressive response of single yeast cells (Saccharomyces cerevisiae), helping determine important characteristics like burst force and burst energy.
- Recent findings illustrate that temperature affects yeast cell strength, with increased temperatures up to 35°C reducing strength, while temperatures up to 50°C significantly enhance burst force and energy, aiding in optimizing conditions for biotechnological processes.