Risks of transmitting ruminant spongiform encephalopathies (prion diseases) by semen and embryo transfer techniques.

Early experiments suggested that scrapie transmission via sheep embryos was a possibility, and gave rise to much controversy. However, when account is taken of the complex genetic effects on ovine susceptibility to scrapie, and of the several different scrapie strains with different clinical and pathological effects, the overall conclusion now is that transmission of classical scrapie by embryo transfer is very unlikely if appropriate precautions are taken. Recent embryo transfer studies have confirmed this.

Historical perspectives on long distance transport of animals.

Since Roman Antiquity, domestic and wild animals have been transported over long distances for purposes as different as improvement of livestock production, food supply, scientific interest, public entertainment, war and numerous other purposes. This long distance transportation was originally limited to the Mediterranean area but, during the Middle Ages extended to the rest of Europe. The conquest of the New World was the first major occasion to transport large numbers of horses and other livestock across the oceans.

Retrospective diagnosis of bluetongue virus in stored frozen and fixed tissue samples using PCR.

Stored frozen (-70 degrees C) and formalin-fixed tissue samples constitute a valuable resource for retrospective studies of infectious diseases, or for diagnostic investigations. The polymerase chain reaction (PCR) affords an accurate and rapid method for detection of viral nucleic acids. It was applied to stored tissue samples collected from sheep inoculated with two Australian serotypes of bluetongue virus, BTV 1 and 23, and two North American serotypes, BTV 11 and 17.