Publications by authors named "I M Krepostnova"
[EFFECT OF OSMOTIC AND OXIDATIVE STRESS ON STRAINS OF GENOVARIANTS OF VIBRIO CHOLERAE EL TOR BIOVAR].
Zh Mikrobiol Epidemiol Immunobiol
March 2016
Article Synopsis
- The study investigates how osmotic and oxidative stress affect the survivability and characteristics of genovariant strains of V. cholerae El Tor biovar.
- Researchers used 22 strains in various conditions, analyzing their phenotypic properties and surface structures, while conducting PCR for genetic analysis.
- Findings showed that genovariants had significantly higher survivability under stress compared to typical strains, partly due to extra protective layers and changes in mobility and genetic elements, suggesting that these features contribute to their enhanced resilience in different environments.
The conjugative recombinant plasmid pIEM3 (KmR TcR) was constructed in order to introduce the cloned ctxB gene encoding the cholera toxin B subunit into the Vibrio cholerae cells. The plasmid was obtained as a result of co-integration of two plasmids: a conjugative plasmid, pIEM1(KmR), carrying mini-kan transposon and IS1 element, as well as the pCTdelta27(TcR) plasmid that is a derivative of the pBR322 which carries the cloned ctxB gene. The avirulent Vibrio cholerae strain eltor biovar deprived (according to the PCR analysis) of the key structural and regulatory pathogenicity genes and carrying a mutation in a single gene of the O1 antigen was chosen as the pIEM3 plasmid carrier strain.
View Article and Find Full Text PDF