Publications by authors named "I Lieberwirth"
Article Synopsis
- * Plunge-freezing samples at higher temperatures presents challenges such as condensation and maintaining stable temperatures, which can affect results.
- * The paper proposes solutions to reduce condensation and temperature fluctuations by preheating equipment and controlling humidity, with successful applications shown using poly(N-isopropylacrylamide) microgels that react to temperature changes.
The objective of this study was to develop two lipid nanoparticle (LNP) formulations capable of efficiently expressing a reporter mRNA while co-delivering the anti-inflammatory drug dexamethasone (DX) to reduce inflammatory side effects in protein replacement therapies. Two types of LNPs were developed, in which 25% of cholesterol was replaced by DX. These LNPs contained either 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) or 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) as a helper lipid.
View Article and Find Full Text PDFChaperone-Derived Copper(I)-Binding Peptide Nanofibers Disrupt Copper Homeostasis in Cancer Cells.
Angew Chem Int Ed Engl
December 2024
Copper (Cu) is a transition metal that plays crucial roles in cellular metabolism. Cu homeostasis is upregulated in many cancers and contributes to tumorigenesis. However, therapeutic strategies to target Cu homeostasis in cancer cells are rarely explored because small molecule Cu chelators have poor binding affinity in comparison to the intracellular Cu chaperones, enzymes, or ligands.
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- The study investigates the relationship between phosphate levels in aqueous humor and the risk of calcification in intraocular lenses (IOLs), especially in diabetic patients.
- In vitro tests involved different IOL models exposed to varying phosphate concentrations to assess calcification using several analytical methods.
- Results indicated that higher phosphate concentrations and longer exposure times significantly increased the risk of IOL calcification, supporting the hypothesis that diabetic conditions may elevate this risk.
Lipid nanoparticles (LNPs) tailored for mRNA delivery were optimized to serve as a platform for treating metabolic diseases. Four distinct lipid mixes (LMs) were formulated by modifying various components: LM1 (ALC-0315/DSPC/Cholesterol/ALC-0159), LM2 (ALC-0315/DOPE/Cholesterol/ALC-0159), LM3 (ALC-0315/DSPC/Cholesterol/DMG-PEG2k), and LM4 (DLin-MC3-DMA/DSPC/Cholesterol/ALC-0159). LNPs exhibited stability and homogeneity with a mean size of 75 to 90 nm, confirmed by cryo-TEM and SAXS studies.
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