[The immunobiological properties of a corpuscular pertussis vaccine inactivated by gamma irradiation].

As a result of the high-dose gamma-irradiation (50 kGy) of corpuscular pertussis vaccine (CPV), pertussis radiovaccine (PRV) was obtained. Compared with CPV, PRV was shown to possess reduced toxicity and higher protective potency. Its adjuvant properties remained at the initial level, while its leukocytosis-promoting and histamine-sensitizing activities drastically decreased.

[The standardization of the in-vitro tetanus toxin neutralization test on Chinese hamster ovary cells].

A test for the titration of B. pertussis toxin with antisera on Chinese hamster ovary (CHO) cells has been worked out. B.

[The phenotypic properties of freshly isolated strains of Bordetella].

As the result of our investigations, newly isolated B. pertussis and B. bronchiseptica strains were studied.

[Structural organization of a segment of chromosome, containing the vir gene of Bordetella pertussis].

To study the structural arrangement of the chromosomal region containing vir genes of Bordetella pertussis the corresponding 15 kb fragment of Bordetella pertussis chromosomal DNA has been cloned. The sequence homology to an earlier characterized Bordetella pertussis genetical element RSBP1 and flanked by two 400 bp inverted repeats has been shown to be located at an end of a BamHI fragment. The restriction map of Bordetella pertussis 475 coincides with the previously published maps of Bordetella pertussis Tohama and 18323 permitting one to conclude the definite conservatism of the cloned sequence.