Effect of Stress on the Production of Antibodies and IL-2, IL-4, and IFNγ Depending on the Time of Antigen Administration and Evaluation of the Role of Opioid Receptors.

The time of stress exposure relative to the moment of immunization affects the direction of the immunoregulatory effect of stress. In case of stress exposure preceding immunization, rotation stress stimulated the production of antibodies, while immobilization depressed it. After antigen injection, these types of stress had no significant effect on the formation of antibody-producing cells.

Chronic cold stress modulates the function of peritoneal macrophages in vivo.

On day 7 of chronic cold stress, concentration of corticosterone in plasma was established to increase, zymosan-induced production of reactive oxygen species was activated, and IL-10 synthesis by macrophages was enhanced regardless of the opiate receptor blockade. Increase in the concentration of corticosterone was independent of the blockade of opioid receptors, whereas the zymosan-induced increase in the production of oxygen radicals and activation of the spontaneous production of IL-10 was blocked in mice by naloxone. Chronic cold stress had no effect on the production of IL-1β and TNF-α.

[INFLUENCE OF COLD STRESS ON FUNCTIONAL ACTIVITY OF MOUSE PERITONEAL MACROPHAGES UNDER OPIATE RECEPTORS BLOCKADE].

The effect of 10- and 60-min cold stress was analyzed on reactive oxygen species (ROS) formation, peritoneal macrophage production of cytokines and corticosterone level under the opiate receptor blockade. It was observed that mice exposed to -20 °C for 10 min demonstrated the ROS inhibition, the effect was leveled against the background of naloxone introduction. The animal group subjected to 60-min stress on the contrary, showed the naloxone-dependent promotion of ROS product.

[Effect of opioid receptor blockade on antibody response and proliferative response under stress conditions].

It is established that rotation and immobilization stress produces bidirectional effect on AFC number in spleen and on proliferative response of splenocytes. The rotation stress results in augmentation of AFC formation and promotion of spontaneous proliferative activity of splenocytes. The micro-opioid receptor blockade with naloxone, but not delta-receptor blockade with naltrindole, abolished the effects of rotation.