Intermetallic Compound and Solid Solutions of Co75Me25 (Me: Si, Fe, Cr) as Catalysts for the Electrochemical Reaction of Nitrate Conversion to Ammonia.

A sustainable reaction of electrocatalytic nitrate conversion in ammonia production (NORR) occurring under ambient conditions is currently of prime interest, as well as urgent research due to the real potential replacement of the environmentally unfavorable Haber-Bosch process. Herein, a series of electrocatalysts based on two-component cobalt alloys was synthesized using low-cost non-noble metals Co, Fe, Cr, and also Si. The samples of electrocatalysts were characterized and studied by the following methods: SEM, EDX, XRD (both transmission and reflection), UV-VIS spectroscopy, optical microscopy, linear (and cyclic) voltammetry, chronoamperometry, and electrochemical impedance spectroscopy.

Myofibroblasts emerge during alveolar regeneration following influenza-virus-induced lung injury.

Alveolar regeneration requires the coordinated engagement of epithelial stem cells and mesenchymal niche cells to restore the intricate alveolar architecture of the lung. The current paradigm is that certain aspects of lung organogenesis are mimicked during injury repair in the adult stage. Here, we employ a longitudinal single-cell transcriptomic survey to fate map lung mesenchymal cells throughout development and adulthood.

Influence of Lamb Wave Anisotropy on Detection of Water-to-Ice Phase Transition.

An important technical task is to develop methods for recording the phase transitions of water to ice. At present, many sensors based on various types of acoustic waves are suggested for solving this challenge. This paper focuses on the theoretical and experimental study of the effect of water-to-ice phase transition on the properties of Lamb and quasi shear horizontal (QSH) acoustic waves of a higher order propagating in different directions in piezoelectric plates with strong anisotropy.

Pitfalls of Using ANS Dye Under Molecular Crowding Conditions.

The 1-anilino-8-naphthalenesulfonate (ANS) fluorescent dye is widely used in protein folding studies due to the significant increase in its fluorescence quantum yield upon binding to protein hydrophobic regions that become accessible during protein unfolding. However, when modeling cellular macromolecular crowding conditions in protein folding experiments in vitro using crowding agents with guanidine hydrochloride (GdnHCl) as the denaturant, the observed changes in ANS spectral characteristics require careful consideration. This study demonstrates that crowding agents can form clusters that interact differently with ANS.

PML Nuclear Bodies and Cellular Senescence: A Comparative Study of Healthy and Premature Aging Syndrome Donors' Cells.

Natural aging and age-related diseases involve the acceleration of replicative aging, or senescence. Multiple proteins are known to participate in these processes, including the promyelocytic leukemia (PML) protein, which serves as a core component of nuclear-membrane-less organelles known as PML nuclear bodies (PML-NBs). In this work, morphological changes in PML-NBs and alterations in PML protein localization at the transition of primary fibroblasts to a replicative senescent state were studied by immunofluorescence.