Publications by authors named "I Kucharska"
Circulating sexual stages of ) can be transmitted from humans to mosquitoes, thereby furthering the spread of malaria in the population. It is well established that antibodies can efficiently block parasite transmission. In search for naturally acquired antibodies targets on sexual stages, we established an efficient method for target-agnostic single B cell activation followed by high-throughput selection of human monoclonal antibodies (mAbs) reactive to sexual stages of in the form of gametes and gametocyte extracts.
View Article and Find Full Text PDFArticle Synopsis
- Connexins (Cxs) are integral membrane proteins that form hemichannels and gap junctions, enabling communication between cells and their environment, which is vital for development and response to diseases.
- Abnormal functioning of these channels can lead to various health issues, including inflammation, skin diseases, deafness, and heart problems.
- Recent studies using high-resolution imaging techniques have revealed detailed structures of Cxs, uncovering mechanisms of channel regulation and potential future research directions to explore their functions in cellular communication.
Article Synopsis
- Researchers used cryo-electron microscopy (cryoEM) to analyze the structure of the adenosine A receptors (AAR) when bound to the G protein Gs, revealing notable differences compared to previous models.
- The binding site of AAR-Gs showed similarities to earlier structures, but the presence of Gβ created a larger interior space within the complex, leading to fewer interactions with the Gs α protein.
- Gβγ was found to be significantly more effective in facilitating AAR-dependent GTPγS binding compared to its analog, suggesting that Gβ may enhance Gs α's activity through positive allosteric modulation.
Unlabelled: Circulating sexual stages of can be transmitted from humans to mosquitoes, thereby furthering the spread of malaria in the population. It is well established that antibodies (Abs) can efficiently block parasite transmission. In search for naturally acquired Ab targets on sexual stages, we established an efficient method for target-agnostic single B cell activation followed by high-throughput selection of human monoclonal antibodies (mAbs) reactive to sexual stages of in the form of gamete and gametocyte extract.
View Article and Find Full Text PDFSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), has been responsible for a global pandemic. Monoclonal antibodies (mAbs) have been used as antiviral therapeutics; however, these therapeutics have been limited in efficacy by viral sequence variability in emerging variants of concern (VOCs) and in deployment by the need for high doses. In this study, we leveraged the multi-specific, multi-affinity antibody (Multabody, MB) platform, derived from the human apoferritin protomer, to enable the multimerization of antibody fragments.
View Article and Find Full Text PDF