Isolation and characterization of rat tubular basement membrane.

Tubules were isolated by the passage of coarsely homogenized kidney parenchyma through a series of stainless steel sieves of varying pore sizes. The isolated tubules, which were free of glomeruli or any contaminating tissue, based on light microscopic examination, served as starting material for the isolation of tubular basement membrane (TBM). The tubules were sonically disrupted, and the subsequent isolation procedure was conceptually similar to that of the isolation of glomerular basement membrane (GBM).

The spontaneous release of 51Cr from labeled EL-4 ascitic lymphoma cells as dependent upon the composition of the cell population.

Investigations on the spontaneous release of 51Cr from the EL-4 ascitic lymphoma showed that daily rhythmic changes can be correlated with the dominance of certain cell types in the population. A generation time of 9.8 h, an average doubling time of 32 h, and an 'appearance cycle' of 96 h were established for the population.

Biosynthetic studies with isolated kidney glomeruli.

Glomeruli were isolated from rat kidneys and were found active in protein and glycoprotein synthesis in vitro. The incorporation of proline, galactose and fucose into macromolecules was linear for at least 8 h. The intracellular pool of free proline and lysine was 52 and 32 nmol/mg glomerular protein respectively.