Combined application of alpha-tocopherol and FC-43 perfluorocarbon emulsion suppresses early postburn lipid peroxidation and improves deformability of erythrocytes.

The effect of FC-43 perfluorocarbon emulsion and alpha-tocopherol on lipid peroxidative damage and deformability of erythrocytes was evaluated in rats (full skin thickness burns over 15-20% of total body surface) at third hour after burns. The animals were divided into five groups: (1) non-burnt non-treated (controls); (2) burnt non-treated; (3) burnt but treated with alpha-tocopherol ("Serva", Germany, 20 mg/kg b.m.

Suppressive effect of FC-43 perfluorocarbon emulsion on enhanced oxidative haemolysis in the early postburn phase.

The effect of FC-43 perfluorocarbon emulsion on resistance of red blood cells to oxidative haemolysis and lipid peroxidation was evaluated in rats (full skin thickness burns over 15-20 per cent of total body surface area). The content of erythrocyte malonyl dialdehyde (MDA), alpha-tocopherol, glutathione (reduced and oxidized forms) and oxidative haemolysis were measured at 24 h after burn injury. Four groups were employed: (1) non-burned non-treated, (2) non-burned but treated with FC-43 perfluorocarbon emulsion (5 ml/kg bodymass i.

Reduced erythrocyte deformability related to activated lipid peroxidation during the early postburn period.

The role of lipid peroxidation in reduced red cell deformability has been examined after thermal injury of rats (full skin thickness over 15-20 per cent of total body surface). An increased concentration of erythrocyte malonyldialdehyde (MDA) and blood thiobarbituric acid (TBA) reactive product levels during the first 3 days after thermal trauma was established. There was a remarkable decrease in red cell superoxide dismutase (SOD) activity after burns and an increase in glucose-6-phosphate dehydrogenase (G-6-PD) on day 3 postburn.

[The correlation between the activity of free-radical peroxidation and erythrocyte hemolysis in experimental thermal trauma].

Increased concentration of the products of red-cell free-radical oxidation (FRO), i.e. of malondialdehyde (MDA) and of Schiff bases in red cells and of their hemolysis was recorded in the acute period of experimental thermic injury.

[Changes in the osmotic resistance of the erythrocytes in thermal trauma and the role of free-radical oxidation].

Reduced erythrocyte osmotic resistance against the background of elevated blood level of TBA-reactive products was recorded in experimental thermic injury (of IIIa-IIIb degree, involving 15-20 per cent of the body surface). Under the influence of alpha-tocopherol, parallel with the restriction of free-radical oxidation (FRO) activity, there was a rise in erythrocyte membrane osmotic resistance. FRO participation in the mechanisms of reduced erythrocyte osmotic resistance in thermic injury is discussed.