Dual Roles of Extracellular Histone H3 in Host Defense: Its Differential Regions Responsible for Antimicrobial and Cytotoxic Properties and Their Modes of Action.

Extracellular histones play a dual role-antimicrobial and cytotoxic-in host defense. In this study, we evaluated the antimicrobial and cytotoxic activities of histone H3 and identified the responsible molecular regions for these properties. Broth microdilution assays indicated that histone H3 exhibits growth inhibitory activity against not only Gram-negative and -positive bacteria but also fungi.

Electronic state dependence of the ion-molecule reaction CH(3)CN(+) + CH(3)CN → CH(4)CN(+) + CH(2)CN: threshold electron-secondary ion coincidence (TESICO) and direct ab initio molecular dynamics study.

The ion-molecule reaction, CH(3)CN(+) + CH(3)CN → CH(3)CNH(+) + CH(2)CN, has been investigated using the threshold electron-secondary ion coincidence (TESICO) technique. Relative reaction cross sections for two microscopic reaction mechanisms, i.e.

Breakdown of the two-step model in K-shell photoemission and subsequent decay probed by the molecular-frame photoelectron angular distributions of CO2.

We report results of measurements and of Hartree-Fock level calculations of molecular-frame photoelectron angular distributions (MFPADs) for C 1s photoemission from CO2. The agreement between the measured and calculated MFPADs is on average reasonable. The measured MFPADs display a weak but definite asymmetry with respect to the O+ and CO+ fragment ions at certain energies, providing evidence for an overlap of gerade and ungerade final ionic states giving rise to a partial breakdown of the two-step model of core-level photoionization and its subsequent Auger decay.

Internal inelastic scattering satellite probed by molecular-frame photoelectron angular distributions from CO2.

The molecular-frame photoelectron angular distribution (MFPAD) of the satellite accompanying the C 1s photoline of the CO2 molecule has been measured at the C 1s(2sigmag)-->4sigmau* shape resonance, using electron-ion multicoincidence momentum spectroscopy. The observed MFPAD indicates that the conjugate satellite is excited by internal inelastic scattering. In this scenario, a photoelectron is ejected from the C 1s(2sigmag) orbital along the molecular axis and collides with an O lone-pair electron in the highest occupied molecular orbital 1pig.

Angular distribution of different vibrational components of the X and B states reached after resonant Auger decay of core-excited H2O: experiment and theory.

Vibrationally resolved spectra have been obtained for the lowest-lying cationic states X (2)B(1), A (2)A(1), and B (2)B(2) of the water molecule reached after participator resonant Auger decay of core-excited states. The angular distribution has been measured of the first four vibrational components of the X state in the photon energy regions including the O 1s-->4a(1) and the O 1s-->2b(2) core excitations, and for different portions of the vibrational envelope of the B state in the photon energy region including the O 1s-->2b(2) core excitation. For the X state, a large relative spread in beta values of the different vibrational components is observed across both resonances.