Publications by authors named "I Kanevsky"
The Golden Syrian hamster is a well-characterized rodent model for severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2)-associated pneumonia. We sought to characterize the pulmonary disease course during SARS-CoV-2 infection (strain USA-WA1/2020) in the hamster model using micro-computed tomography (micro-CT) and compare radiologic observations with histopathologic findings. We observed a range of radiologic abnormalities, including ground glass opacities (GGOs), consolidations, air bronchograms, and pneumomediastinum.
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- - A new method called B3E-seq allows for the recovery of full-length variable region sequences of B cell receptors (BCRs) from commonly used 3'-barcoded single-cell RNA sequencing (scRNA-seq) libraries, addressing a gap in current techniques.
- - The accuracy of B3E-seq was verified, and it was used to study B cell responses from vaccinated infant rhesus macaques against the Streptococcus pneumoniae serotype 3 (ST3) capsular polysaccharide.
- - The study found common BCR features associated with the ST3 antigen in multiple monkeys, suggesting a similar immune response to the glycoconjugate vaccines, highlighting the method's potential for analyzing antigen
The increase in urinary tract infections (UTI) caused by antibiotic-resistant requires the development of new therapeutic agents and prophylactic vaccines. To evaluate the efficacy of new lead candidates, we implemented a cynomolgus macaque UTI challenge model that mimics human uncomplicated cystitis in response to transurethral challenge with a multidrug-resistant (MDR) serotype O25b ST131 isolate. fimbrial adhesin FimH and O-antigens are separately under clinical evaluation by others as vaccine candidates to prevent UTI and invasive urosepsis disease, respectively.
View Article and Find Full Text PDFBackground: Vaccine candidate VLA15 is designed to protect against the dominant genospecies-causing Lyme disease in North America and Europe. Active immunization with VLA15 has protected in the mouse model of tick challenge. VLA15 is currently under evaluation in clinical studies for the prevention of Lyme borreliosis.
View Article and Find Full Text PDFRecent phylogenetic profiling of pneumococcal serotype 3 (Pn3) isolates revealed a dynamic interplay among major lineages with the emergence and global spread of a variant termed clade II. The cause of Pn3 clade II dissemination along with epidemiological and clinical ramifications are currently unknown. Here, we sought to explore biological characteristics of dominant Pn3 clades in a mouse model of pneumococcal invasive disease and carriage.
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