Specific cleavage of hybrid proteins by proteinase encoded by the KEX2 gene.

A method for isolation of the KEX2-gene-encoded membrane-bound proteinase from alpha-cells of Saccharomyces cerevisiae yeast has been modified. The isolated enzyme hydrolyzes peptides and proteins with basic amino acid pairs which are cleaved at the C-ends of their peptide bonds. Because KEX2 proteinase is located within the Golgi compartment, it may be isolated by differential centrifugation of broken cells at 7000g for 15 min and at 20,000g for 15 min.

[The diagnosis of a cystic duct blockade in patients with chronic cholecystitis].

The authors describe a method of complex examination of patients with diseases of the external biliary tract with the aim of diagnosing cystic duct blocks. Intravenous cholangiography using billignost and albumin in 2:1 ratio proved highly effective. Of essential help in the diagnosis of cystic duct block was dynamic ultrasonic examination.

[Functional state of the stomach after gastrectomy and vagotomy and the ways of improving the results of surgical treatment of peptic ulcer].

A complex examination of 286 patients was performed after gastric resection by the method of Hofmeister-Finsterer, 64 patients after truncal vagotomy with antrum-resection, 14--after selective proximal vagotomy with draining operations and 26--without draining operation. Time for the recovery of motor-evacuatory function of the stomach after organ-preserving operations was considerably shorter than after resection of the stomach. In order to shorten the period of adaptation of the digestion organs after operative treatment of ulcer disease a complex of rehabilitating measures is proposed including percutaneous electrostimulation, diet, balneological and medicamentous therapy.