[Types of Y chromosome deletions and their frequency in infertile men].

Deletions of Y chromosome AZF locus were analyzed during a large-scale andrological and genetic examination of 810 infertile men. The search for Yq microdeletions was carried out according to the standard EAA/EMQN guidelines. The breakpoints were mapped for the deletions in AZF locus.

[Expression of recombinant DNA RSVlacZ and pCMVlacZ in preimplantation rabbit embryos].

A linear fragment of the pRK3lacZ plasmid (RSVlacZ) and circular DNA of pCMVlacZ were injected into rabbit zygotes. The embryos were cultured for 24-72 h and analyzed with chromogenic X-gal substrate for bacterial beta-galactosidase. The expression of RSVlacZ DNA was revealed in 6-16-cell embryos.

[Polymorphism of untranscribed spacer rDNA as a molecular genetic marker in population studies of cattle].

Variable polymorphic patterns were detected using EcoRI-SalI fragment of bovine rDNA, including 3'-end of 28S rRNA gene with the adjacent portion of the transcribed spacer, as a probe for hybridization. Some features of these polymorphic patterns are similar to DNA fingerprints detected with the M13 probe. Bovine rDNA spacer polymorphism was used as a molecular genetic marker for population analysis of individual specific patterns of 4 cattle breeds with the help of the Jeffreys' method.