Publications by authors named "I K Barton"
Article Synopsis
- MucR is a transcriptional regulator found in Brucella species that influences gene expression related to virulence by binding to AT-rich DNA regions.
- MucR is part of the Ros/MucR family in α-proteobacteria and shares functional similarities with H-NS proteins, although they lack sequence homology.
- This study uses cryo-EM and other methods to reveal that MucR and its homolog Ml5 form a unique circular structure that can condense DNA, linking nucleoid structure to transcription regulation.
Bacteria rely on DNA methylation for restriction-modification systems and epigenetic control of gene expression. Here, we use direct detection of methylated bases by nanopore sequencing to monitor global DNA methylation in Alphaproteobacteria, where use of this technique has not yet been reported. One representative of this order, , relies on DNA methylation to control cell cycle progression, but it is unclear whether other members of this order, such as , depend on the same systems.
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- MucR is part of a protein family that plays a crucial role in regulating virulence and symbiosis genes in α-proteobacteria.
- Recent research has redefined MucR from a classical transcriptional regulator to a unique Histone-like Nucleoid Structuring (H-NS) protein.
- The review aims to compile genetic and biochemical evidence showing that MucR binds to AT-rich DNA regions, influencing gene expression differently than traditional regulators.
Bacteria rely on DNA methylation for restriction-modification systems and epigenetic control of gene expression. Here, we use direct detection of methylated bases by nanopore sequencing to monitor global DNA methylation in Alphaproteobacteria, where use of this technique has not yet been reported. One representative of this order, , relies on DNA methylation to control cell cycle progression, but it is unclear whether other members of this order, such as , depend on the same systems.
View Article and Find Full Text PDFHistone-like nucleoid structuring (H-NS) and H-NS-like proteins coordinate host-associated behaviors in many pathogenic bacteria, often through forming silencer/counter-silencer pairs with signal-responsive transcriptional activators to tightly control gene expression. and related bacteria do not encode H-NS or homologs of known H-NS-like proteins, and it is unclear if they have other proteins that perform analogous functions during pathogenesis. In this work, we provide compelling evidence for the role of MucR as a novel H-NS-like protein in .
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