Accumulation of sulfate by mitochondria of rat kidney cortex.

Twice washed mitochondria from rat kidney cortex can accumulate sulfate ions from low (10(-7)M) ambient concentrations to create virtual gradients of several hundred to one. This sulfate is subsequently released. The activation energy for the uptake is 12,000 calories per mole; for release it is about 30,000 calories per mole.

The effect of temperature on the uptake of radiosulfate by rat renal tissue from radiosulfate-containing solutions in vitro.

Kidney cortex slices incubated in vitro at 0 degrees C. accumulate radiosulfate from the incubation medium. This process differs from the previously described uptake of radiosulfate by renal tissue incubated at 38 degrees C.

Factors influencing the uptake and loss of radiosulfate by rat renal cortical tissue in vitro.

Rat kidney cortical slices, during incubation in vitro, lose previously accumulated radiosulfur when exposed to conditions (e.g. addition to the medium of metabolic inhibitors) which normally depress the uptake of S(35).