[Comparative analysis of LAMP and Real Time PCR methods to detect pathogens of glanders and meliodosis.].

Results of detection of Burkholderia mallei and Burkholderia pseudomallei DNA strains by LAMP (Loop-mediated Isothermal Amplification) and Real Time PCR are shown. It has been revealed that, in Real Time PCR, primers steadily detected DNA of those microorganism for the sequences of which they were designed. The above mentioned primers did not detect DNA of heterologous strains.

[The loop mediated isothermal amplification of DNA: principle of method and perspectives of application in molecular diagnostic of cholera: publications review].

The article considers characteristics of technology of reaction of loop mediated isothermal amplification of DNA (LAMP), issues of optimization of reaction and perspectives of its application as a quick highly-specific test in molecular diagnostics of infectious diseases and monitoring of contamination of environment objects with pathogens. The analysis of publications data concerning application of LAMP in diagnostics of cholera testifies high diagnostic value. The LAMP supports possibility of direct rapid detection of toxin-producing strains of Vibrio cholerae in clinical samples.

The use of loop-mediated isothermal DNA amplification for the detection and identification of the anthrax pathogen.

The results of detection and identification of strains in loop-mediated isothermal DNA amplification (LAMP) reaction performed under optimized conditions with original primers and thermostable DNA polymerase are presented. Reproducible LAMP-based detection of chromosomal and plasmid DNA targets specific for strains has been demonstrated. No cross reactions with DNA from bacterial strains of other species of the group were detected.

[Detection of Babesia canis (Piroplasmida) DNA in the blood samples and lysates of the ticks Dermacentor reticulatus (Ixodidae) collected in the Tula and Moscow Regions].

Chimeric primers, the sensitivity and specificity of which allow them to be used in both the clinical setting and the epizootological assessment of tick infection by a real-time polymerase chain reaction (RT-PCR) assay, have been designed against Babesia canis infection. The findings suggest that a large number of Babesia DNA copies are detectable in the blood in acute babesiosis. Some animals that had experienced babesiosis developed blood B.

[Application of multiplex PCR for analysis of polycomponent probiotic preparations].

Aim: Development of multiplex PCR for indication and differential analysis of CFU in a probiotic preparation containing 3 various species ofbifidobacteria (Bifidobacterium bifidum, B. longum, B. adolescentis) and 2 species of lactobacilli (Lactobacillus acidophilus, L.