[Effect of adenosine protonation on its interaction with polyuridylic acid].

The effect of adenosine protonation on complex formation between poly(U) and adenosine has been studied by UV spectroscopy, titration and equilibrium dialysis techniques. A method has been developed to estimate the "misincorporation" of ionized monomer molecules into a polynucleotide--monomer complex. The method is based on combining the titration and binding data.

[Effect of ionization of system components on the interaction of polyuridylic acid with adenosine. Deprotonation of the polynucleotide].

The interdependence of complex formation and poly(U) deprotonation in the poly(U)-adenosine system has been studied by UV spectroscopy, titration and equilibrium dialysis techniques. Evidence is presented on the formation of an intermediate, probably double helical, structure in the course of A-2 poly(U) dissociation at alkaline pH.