[Method of preparation of tissue engineering and cell cultivation collagen by acid extraction of calf skin].

Seven methods of preparation of intact native collagen with telopeptides by acid extraction of calf skin have been compared; the hide was first dehaired by original mild enzymatical method using Bacillus licheniformis protease. The noncollagenous proteins and proteoglycans were previously removed by different ways and collagen was extracted by acid solvents and purified by salt precipitation. The dynamic of noncollagen impurities removing was followed by noncollagen proteins and hexuronic acids analysis in extracts.

[Degradation of alkylsulfates by a Pseudomonas aeruginosa culture immobilized on a polyvinyl alcohol fiber].

Pseudomonas aeruginosa cells capable of destroying alkyl sulfates, anionic surfactants, were immobilised on activated polyvinyl alcohol fibres. The immobilised cells could decompose SDS. When the immobilised cells were used repeatedly, their biomass increased but the activity hardly changed.

[Immobilized papain in the treatment of acute destructive lactation mastitis].

Experience with the surgical treatment of 123 patients has shown the positive effect of proteolytic enzyme Papain immobilized on fiber polymer materials. It shortened the period of purification of purulent wounds of the lactating mammary gland from devitalized tissues which facilitated putting early secondary sutures on the granulating wound without a dissection of wound margins and resulted in quicker healing (13,5 days).