Unlabelled: The aim of this study was to investigate the reaction of normal and tumor cells to genotoxic effect of widespread environmental factors - exogenous nitric oxides and ionizing radiation.
Methods: The animals were treated with NO (125 mg/m(3)) and low dose ionizing radiation (10 acute exposures with 0.1 Gy each).
Probl Radiac Med Radiobiol
February 2015
Objective: to investigate the effects of a prolonged influence of nitric oxide and low dose ionizing radiation on the blood system.
Materials And Methods: Adult random-bred male rats 120-150 g of body weight. Animals were treated with different factors: inhalation of nitric oxide for 1 month (14 h per day), fractionated X-ray irradiation (1.
Unlabelled: The aim of this study was to investigate impact of exogenous nitric oxide (NO) on generation of different types of DNA damages, their transformation, and specificity of DNA repair in cells treated with ionizing radiation (IR).
Methods: levels of single-strand and double-strand breaks assessed in peripheral blood lymphocytes (PBL) isolated from healthy humans and treated in vitro with NO donor -- S-nitrosoglutathione (GSNO) and IR. The rate of DNA repair estimated after 30 and 60 min of PBL treatment.
Unlabelled: The aim of this study was to investigate the ability of environmental nitrogen oxides or natural nitric oxide (NO) donors to modify free radicals ba-lance and development of genomic instability alone or in combination with ionizing radiation.
Methods: Genotoxicity and cytogenetic abnormalities were assessed in vitro in peripheral blood lymphocytes (PBL) isolated from healthy humans or in vivo in rats PBL. Human PBL were treated with physiologically relevant NO donor - S-Nitrosoglutathione and X-ray irradiation.
Peculiarities of repair of DNA injuries induced by radiation and hyperthermia and their realization on chromosomal level in bone marrow cells ofexperimental animals with different radiation sensitivity were studied. It is shown that radiomodification efficiency of mild hyperthermia is higher for radioresistant animals. More intensive elimination of chromosomal injuries the level of which in remote terms of examination corresponds to the control value, is observed.
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