A novel approach to encode melodies in DNA.

DNA data storage has gained more attention last decades. DNA molecules can be used for encoding of non-biological information and as promising carriers due to greater data capacity, higher duration of the storage, and better technical failures stability. Here we propose a new method for encoding of notes and music in DNA.

LAMPrimers iQ: New primer design software for loop-mediated isothermal amplification (LAMP).

Nucleic acids amplification is a widely used technique utilized for different manipulations with DNA and RNA. Although, polymerase chain reaction (PCR) remains the most popular amplification method, isothermal approaches are gained more attention last decades. Among these, loop-mediated isothermal amplification (LAMP) became an excellent alternative to PCR.

Interplay of Conformational and Chemical Transformations of Ortho-Substituted Aromatic Nitroso Oxides: Experimental and Theoretical Study.

The mechanism of the photooxidation of aromatic azides containing a substituent at one of the ortho positions (2,4-dimethoxyphenyl azide (1a) and 2-methyl-4-[(2E)-1-methylbut-2-en-1-yl]phenyl azide (1b)) was studied in acetonitrile. The electronic spectra and the kinetic regularities of the consumption of corresponding nitroso oxides, which are the reaction intermediates, were investigated by flash photolysis. Owing to the one-and-a-half order of the C-N and N-O bonds and asymmetric molecule structure these nitroso oxides exist as four conformers (cis/syn, cis/anti, trans/syn, and trans/anti).

The Modified Heparin-Binding L-Asparaginase of Wolinella succinogenes.

The modified asparaginase Was79 was derived from the recombinant wild-type L-asparaginase of Wolinella succinogenes. The Was79 contains the amino acid substitutions V23Q and K24T responsible for the resistance to trypsinolysis and the N-terminal heparin-binding peptide KRKKKGKGLGKKR responsible for the binding to heparin and tumor K562 cells in vitro. When tested on a mouse model of Fischer lymphadenosis L5178Y, therapeutic efficacy of Was79 was significantly higher than that of reference enzymes at all single therapeutic doses used (125-8000 IU/kg).