[Serine proteinase from the archaebacterium Halobacterium mediterranei--an analog of eubacterium subtilisin].

A homogeneous serine proteinase was isolated from cultural filtrates of the extreme halophilic bacteria Halobacterium mediterranei 1538 using affinity chromatography on bacitracin-Sepharose, ultrafiltration and gel filtration on Sephadex G-75, with a 48% yield and 260-fold purification. The enzyme was completely inactivated by specific inhibitors of serine proteinases, PMSF and DFP, as well as by Hg2+ and PCMB. The enzyme activity was strongly dependent of NaCl concentration, the enzyme being inactivated below 0.

[Diagnosis and control of the treatment of patients with precancerous conditions of the endometrium using transvaginal echography].

Twenty-six women aged 41 to 73 with clinical and morphologic forms of endometrial precancer were examined by transvaginal ultrasonic scanning. This method was employed both in the diagnosis and monitoring of the efficacy of hormone therapy. The final diagnosis was made on the basis of histologic examination of endometrial scrapings-off.

[Results of the treatment of infertility by the method of extracorporeal fertilization].

Outpatient in vitro insemination has been performed in 609 infertile couples in 1145 menstrual cycles. Age of the women ranged from 19 to 44 years and their history of infertility, from 2 to 17 years. Tubal infertility was present in 439 patients and one or both tubes were patent in 170 patients.