Antigen-induced contraction of human isolated lung preparations passively sensitized with monoclonal IgE: effects of indomethacin.

Human isolated lung preparations were passively sensitized using mouse monoclonal dinitrophenyl (DNP)-specific IgE antibodies. The contractile response to antigen (DNP-bovine serum albumin; DNP-BSA) was approximately 80% of the histamine response (50 microM: 0.20 +/- 0.

Biosynthesis of paf-acether. Paf-acether but not leukotriene C4 production is impaired in cultured macrophages.

After adherence for 24 or 48 h mouse peritoneal macrophages, upon a zymosan challenge, synthesized 114 +/- 55 and 82 +/- 31 pmol of paf-acether (paf)/mg of protein respectively, as compared with 513 +/- 195 pmol of paf/mg of protein in 2 h-adherent macrophages (means +/- S.D., n = 10).

Antigenic contraction of guinea pig tracheal preparations passively sensitized with monoclonal IgE: pharmacological modulation.

Spirally cut guinea pig tracheal preparations were passively sensitized using a mouse monoclonal IgE antibody against dinitrophenol (DNP). Maximal contraction observed following challenge with DNP-bovine serum albumin (DNP-BSA, 5 micrograms/ml; n = 20) response was approximately 46% of the histamine response (0.52 +/- 0.

Platelet-activating factor-acether-induced relaxation of guinea pig airway muscle: role of prostaglandin E2 and the epithelium.

A fixed concentration of paf-acether (platelet-activating factor; 4 microM) relaxed isolated guinea pig tracheal preparations which had been contracted with histamine (50 microM), serotonin (1 microM) or leukotriene D4 (0.1 microM). The relaxations were approximately 43, 100 and 57%, respectively.

Relaxation of isolated human pulmonary muscle preparations with prostacyclin (PGI2) and its analogs.

The effects of PGI2 and two analogs Iloprost and ZK 96480 were examined on isolated human pulmonary muscle preparations. High concentrations of these agents reduced the basal tone in all types of preparations. In addition, they relaxed tissues which had been maximally contracted with histamine (50 microM).