Physical Pretreatments Applied in Three Commercial Kits for the Extraction of High-Quality DNA from Activated Sewage Sludge.

Obtaining sufficient and high-quality genomic DNA from sludge samples is a fundamental issue of feasibility and comparability in genomic studies of microbial diversity. Commercial kits for soil are often used for the extraction of gDNA from sludge samples due to the lack of specific kits. However, the evaluation of the performance of commercial kits for sludge DNA extraction is scarce and optimization of these methods to obtain a high quantity and quality of DNA is necessary, especially for downstream genomic sequencing.

Controversial Roles of Gut Microbiota-Derived Short-Chain Fatty Acids (SCFAs) on Pancreatic β-Cell Growth and Insulin Secretion.

In the past 15 years, gut microbiota emerged as a crucial player in health and disease. Enormous progress was made in the analysis of its composition, even in the discovery of novel species. It is time to go beyond mere microbiota-disease associations and, instead, provide more causal analyses.

The midwife and her functions by level of care.

The role the midwives play in women's healthcare in Chile demonstrates appropriate utilization of human resources according to their capacity to provide services. Historically, the Chilean midwife came into being more than 160 years ago, exclusively as a birth attendant. The midwives' functions have evolved, particularly during the last few decades, following the demographic and epidemiological evolution of the Chilean population and the new paradigm of healthcare addressed to woman throughout her life cycle, not only during pregnancy and childbirth.