Publications by authors named "I Gigante"
Article Synopsis
- Cancer-associated fibroblasts (CAFs) contribute to the aggressive nature and treatment resistance of intrahepatic Cholangiocarcinoma (iCCA), prompting research into the effects of the Notch1 inhibitor Crenigacestat on this interaction.
- Using both 2D and 3D in vitro models, along with a xenograft setup, the study found that CAFs enhance iCCA cell migration and growth, but Crenigacestat effectively inhibits this cross-talk and reduces tumor growth and fibrosis.
- Overall, targeting the hCAF/iCCA interaction with Crenigacestat presents a promising approach to slowing cancer progression and altering critical cell cycle pathways.
Article Synopsis
- Intrahepatic cholangiocarcinoma (iCCA) is a highly aggressive liver cancer with poor treatment options, prompting research into new therapies targeting the Heat Shock Factor 1 (HSF1) transcription factor.
- Studies showed that HSF1 levels are significantly elevated in various stages of iCCA and correlate with worse patient outcomes, while inhibiting HSF1 delayed tumor development in mouse models.
- The HSF1 inhibitor KRIBB-11 was effective in slowing iCCA cell growth, inducing cell death, and reducing key metabolic functions in cancer cells, highlighting its potential as a therapeutic avenue.
Neurons are highly dependent on mitochondria to meet their bioenergetic needs and understanding the metabolic changes during the differentiation process is crucial in the neurodegeneration context. Several in vitro approaches have been developed to study neuronal differentiation and bioenergetic changes. The human SH-SY5Y cell line is a widely used cellular model and several differentiation protocols have been developed to induce a neuron-like phenotype including retinoic acid (RA) treatment.
View Article and Find Full Text PDFPurpose: To evaluate client satisfaction with prosthetic and orthotic services in Sweden, determine if satisfaction differs between clients using different devices and identify factors which influence client satisfaction.
Materials And Methods: A cross-sectional design was used to survey 7318 clients. The survey included items related to demographics, quality of life, device comfort, device use, the extent to which clients' needs were met and satisfaction with services.
Aggressive hepatocellular carcinoma (HCC) overexpressing Angiopoietin-2 (ANG-2) (a protein linked with angiogenesis, proliferation, and epithelial-mesenchymal transition (EMT)), shares 95% of up-regulated genes and a similar poor prognosis with the proliferative subgroup of intrahepatic cholangiocarcinoma (iCCA). We analyzed the pro-invasive effect of ANG-2 and its regulator vascular endothelial growth factor (VEGF) on HCC and CCA spheroids to uncover posUsible common ways of response. Four cell lines were used: Hep3B and HepG2 (HCC), HuCC-T1 (iCCA), and EGI-1 (extrahepatic CCA).
View Article and Find Full Text PDF