Improvements in slide preparation from archival material for automated DNA measurement by image analysis.

Objective: To optimize slide preparation for DNA content measurement by automated image analysis and to obtain a rapid and easy method for routine use.

Study Design: Some improvements in previously described methods were achieved: automatic dewaxing, reduction of washing times, accurate adjustment of final concentration of nuclei, sedimentation and temperate drying of nuclei.

Results: The complete preparation of 12 slides required four intermittent working hours.