Publications by authors named "I Gaivao"
Article Synopsis
- Intracytoplasmic sperm injection (ICSI) relies on the health of both oocytes and cumulus cells; this study investigates the role of DNA damage in these cumulus cells as it relates to fertilization success.
- Using the alkaline comet assay, researchers compared DNA damage levels in cumulus cells and whole blood from potentially fertile and infertile females, finding significant differences.
- Results indicate that higher DNA damage in cumulus cells correlates with lower fertilization success in females with ovulatory dysfunction, suggesting DNA damage could be a useful biomarker for predicting oocyte competence.
The integrity of the genome is under constant threat from both endogenous and exogenous factors that induce oxidative stress and accelerate ageing. The demand for natural and organic cosmetics is rising due to the harmful effects of synthetic genotoxic agents on human health and the environment. Elderberry ( L.
View Article and Find Full Text PDFLemon essential oil, derived from , possesses diverse health-promoting properties, including antioxidant, antimicrobial, and mood-enhancing effects. Despite its traditional use in aromatherapy and complementary medicine, there is a need for comprehensive investigations into its therapeutic potential, particularly in mitigating DNA damage and supporting health in palliative care settings. This study aimed to evaluate the antigenotoxic effects of lemon essential oil in human peripheral blood mononuclear cells and to explore its potential applications in palliative care.
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- The zebrafish is a popular model organism in research due to its unique features like transparent embryos, a fully sequenced genome, and low maintenance costs.
- The comet assay is a technique used to assess DNA damage in zebrafish cells, involving steps like cell lysis and electrophoresis to visualize DNA strand breaks.
- Results are quantified and classified into five damage levels to calculate the Genetic Damage Index (GDI), which helps in evaluating toxic effects at various biological levels.
The comet assay in Drosophila has been used in the last few years to study DNA damage responses (DDR) in different repair-mutant strains and to compare them to analyze DNA repair. We have used this approach to study interactions between DNA repair pathways in vivo. Additionally, we have implemented an ex vivo comet assay, in which nucleoids from treated and untreated cells were incubated ex vivo with cell-free protein extracts from individuals with distinct repair capacities.
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