Publications by authors named "I G Reidel"
Modified vaccinia Ankara (MVA) virus is a widely used vaccine platform, making accurate titration essential for vaccination studies. However, the current plaque forming unit (PFU) assay, the standard for MVA titration, is prone to observer bias and other limitations that affect accuracy and precision. To address these challenges, we developed a new flow cytometry-based quantification method using a highly specific monoclonal antibody (mAb) for the detection of MVA-infected cells, as a more accurate titration assay.
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- Epstein-Barr virus (EBV) is linked to various cancers and diseases, yet despite extensive research, an effective vaccine has not been licensed, largely due to past efforts focusing on a single protein.
- The study presents a new vaccine, MVA-EBV5-2, targeting five EBV entry glycoproteins, demonstrating genetic stability and the ability to produce strong immune responses in animal models.
- Results showed that this vaccine generated higher levels of neutralizing antibodies in mice and rhesus macaques compared to traditional methods, effectively reducing EBV infection in treated models.
Unlabelled: Kaposi sarcoma-associated herpesvirus (KSHV), also known as human herpesvirus-8, is the causal agent of Kaposi sarcoma, a cancer that appears as tumors on the skin or mucosal surfaces, as well as primary effusion lymphoma and KSHV-associated multicentric Castleman disease, which are B-cell lymphoproliferative disorders. Effective prophylactic and therapeutic strategies against KSHV infection and its associated diseases are needed. To develop these strategies, it is crucial to identify and target viral glycoproteins involved in KSHV infection of host cells.
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- A vaccine works best when it triggers the immune system to fight off germs. Different ingredients called adjuvants help boost this immune response.
- Scientists tested a new type of liposome (a tiny bubble) combined with special molecules to see how well it helps the immune system recognize a specific bacteria, Staphylococcus aureus.
- The results showed that this new mix could help create strong immune defenses in mice, making it a good candidate for future vaccines against tricky germs.
The significant impact of infections worldwide highlights the need to develop a prophylactic vaccine that elicits effective immunity and protects the host from the immunopathological effects of infection. The aim of this study was to evaluate a vaccine based on a fragment of the Polymorphic membrane protein D (FPmpD) of as an immunogen using a heterologous DNA prime-protein boost strategy in female mice Three different formulations were evaluated as protein boost: free recombinant FPmpD (rFPmpD) or rFPmpD formulated with a liposomal adjuvant alternatively supplemented with CpG or a cationic lipopeptide as immunostimulants. The three candidates induced an increase in the cervicovaginal and systemic titers of anti-rFPmpD antibodies in two strains of mice (BALB/c and C57BL/6), with no evidence of fertility alterations.
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