The effects of pH, aeration, and temperature on the growth of fungal strain Mortierella alpina LPM-301 and the synthesis of lipids and arachidonic acid in glycerol-containing medium were studied. Arachidonic acid production in the stationary growth phase was found to depend considerably on the pH value; it reached the optimum at pH 6.0 and was irreversibly inhibited at a pH of 3.
View Article and Find Full Text PDFThe values of gross metabolic flows in cells are essentially interconnected due to conservation laws of chemical elements and interrelations of biochemical coupling. Therefore, the overall stoichiometry of cellular metabolism, such as the biomass quantum yield, the ratio between linear and circular flows via the electron transport chain, etc., can be calculated using balances of metabolic flows in the network branching points and coupling ratios related to ATP formation and expenditures.
View Article and Find Full Text PDFAppl Microbiol Biotechnol
October 2010
Oleaginous yeasts (18 strains) were grown in ethanol media under various cultivation conditions (33 biomass samples). It was found that lipid and lipid-free fractions of dry biomass have elemental composition and biomass reductivity very close to values which can be considered as biological constants. The energy content of dry biomass strongly depended on the total lipid content.
View Article and Find Full Text PDFThe current state of knowledge concerning the unsolved problem of the huge interspecific eukaryotic genome size variations not correlating with the species phenotypic complexity (C-value enigma also known as C-value paradox) is reviewed. Characteristic features of eukaryotic genome structure and molecular mechanisms that are the basis of genome size changes are examined in connection with the C-value enigma. It is emphasized that endogenous mutagens, including reactive oxygen species, create a constant nuclear environment where any genome evolves.
View Article and Find Full Text PDFAppl Microbiol Biotechnol
January 2008
Bacterial strain VKM B-2445 is characterized by ethylenediaminetetraacetate (EDTA) requirement for cell growth. This strain could not grow on glucose and organic acids as the sole sources of carbon and energy, but it was able to metabolize these substrates added to EDTA medium. EDTA initiated assimilation of glucose, succinate, fumarate, malate, and citrate and supplied nitrogen for the biomass production from these substrates.
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