Publications by authors named "I G Hide"
Article Synopsis
- Calphostin C (Cal-C) is a known protein kinase C (PKC) inhibitor that can inhibit PKC activation at low concentrations but activate it at high concentrations through a light-dependent mechanism.
- At low doses (<200 nM), Cal-C successfully stops PKC translocation and activation induced by certain compounds, while at higher doses (>2 μM), it raises intracellular calcium levels by mobilizing the endoplasmic reticulum (ER) without involving specific receptors.
- The study also found that high concentrations of Cal-C cause structural changes in the ER and generate singlet oxygen, which contributes to PKC activation and indicates its potential use in photodynamic therapy.
In this study, we aimed to elucidate the action mechanisms of propofol, particularly those underlying propofol-induced protein kinase C (PKC) translocation. Various PKCs fused with green fluorescent protein (PKC-GFP) or other GFP-fused proteins were expressed in HeLa cells, and their propofol-induced dynamics were observed using confocal laser scanning microscopy. Propofol-induced PKC activation in cells was estimated using the C kinase activity receptor (CKAR), an indicator of intracellular PKC activation.
View Article and Find Full Text PDFMicroglial removal of dying cells plays a beneficial role in maintaining homeostasis in the CNS, whereas under some pathological conditions, inflammatory microglia can cause excessive clearance, leading to neuronal death. However, the mechanisms underlying dying cell removal by inflammatory microglia remain poorly understood. In this study, we performed live imaging to examine the purinergic regulation of dying cell removal by inflammatory activated microglia.
View Article and Find Full Text PDFPropofol is widely used for general anesthesia and sedation; however, the mechanisms of its anesthetic and adverse effects are not fully understood. We have previously shown that propofol activates protein kinase C (PKC) and induces its translocation in a subtype-specific manner. The purpose of this study was to identify the PKC domains involved in propofol-induced PKC translocation.
View Article and Find Full Text PDFThe neurotransmitter serotonin (5-HT) is transported back into serotonergic neurons by the serotonin transporter (SERT). SERT is a main target of antidepressants, and much effort has therefore focused on finding relationships between SERT and depression. However, it is not fully understood how SERT is regulated at the cellular level.
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