[Separation of leghemoglobin from the nodules of lupine, Lupinus luteus L., into components].

The crude leghaemoglobin suspension from root nodules of yellow lupine (Lupinus luteus L.) was separated into five fractions: I, IIa and IIb, IIIa and IIIb using the chromatography on DEAE-cellulose (numbered following the elution order). The visible absorption spectra show that fractions I, IIa and IIIa are met-leghaemoglobins while IIb and IIIb are oxy-forms.