[Frequency and rearrangements of alleles of proto-oncogene c-Ha-ras-1 and their association with development of various malignant tumors in man].

The c-Ha-ras-1 locus in 84 cancer patients was examined for allelic restriction fragment length's polymorphism, as well as for distribution of four common c-Ha-ras-1 alleles (a1, a2, a3 and a4) in lung, ovarian and thyroid cancer patients. In approximately half (8 out of 15) lung and ovarian carcinomas possessing a4 allele, alterations in the Ha-ras locus (deletion, amplification and change in allele length) were detected, as compared to 2 cases of rearrangements out of 40 tumors lacking the a4 allele. An increased a4 allele frequency was found in individuals with lung and ovarian carcinomas, as compared to both controls--summarized literature data, and thyroid cancer patients.

[A molecular genetic analysis of the 12th codon of the Ha-ras-1 proto-oncogene in human carcinoma and stomach ulcer cells].

A method of the restriction analysis by Msp I enzyme has been used to analyze the 12th codon of Ha-ras-1 protooncogene in 10 human carcinomas and in the stomach mucosa adjacent to them their 5 metastases into the regional lymph nodes and in 2 ulcers. No point mutation was found.

[The role of proto-oncogenes in fundamental manifestations of life].

Problems of the origin, structure and functions, in the cells and tissues, of the so-called proto-oncogenes (c-onc)--cell genes, homologues and oncogene foreparents (v-onc)--are considered in the review. Up-to-date data are given to show the participation of the main groups of proto-oncogenes in the processes of cell division, proliferation and differentiation. The role of c-onc genes is found to be important for fundamental manifestations of the cell life activity.

[Polymorphism of restriction fragments of the Ha-ras-1 protooncogene in patients with carcinoma and ulcers of the stomach].

Ha-ras restriction fragments' length polymorphism (RFLP) in white blood cells and stomach tissues from patients with carcinoma and ulcer of the stomach was examined. Genomic DNAs were digested with Xho I, Pvu II, Pst I, Msp I, Bcn I, Mva I, Bsp RI. No Ha-ras-1 polymorphic variants specifically associated with the cancer disease were detected.

[Can the presence of an RNA-lipoprotein complex in human blood serum give evidence of a cancerous disease?].

The fractional composition of serum lipoproteids in patients with malignant tumours and in noncancerous disorders as well as in healthy donors has been investigated to detect the cancer specificity of the appearance of the additional abnormal RNA-lipoprotein fraction. No distinct correlation is found between the presence of the additional serum lipoprotein fraction and human malignancy.