What DNA Polymerase Is Preferable in miRNA Assay Coupled with Isothermal Circular Strand Displacement Polymerization Reaction (ICSDPR)?

The products generated in the isothermal circular strand displacement polymerization reaction (ICSDPR) initiated with miRNA-141 were studied. The obtained results demonstrated that if ICSDPR was catalyzed with Large Klenow Fragment (lKF), the canonical duplex and some byproducts, which were not described previously, were observed. The HMW byproducts were shown to be produced as a result of lKF-insisted polymerization of capture hairpin (HP) used in ICSDPR.

Chemiluminescent plate assay of microRNA-155 coupled with catalytic hairpin assembly with oligonucleotide release (CHAOR).

A heterogeneous sensitive microRNA-155 assay based on a new isothermal amplification method, called catalytic hairpin assembly with oligonucleotide release (CHAOR), was developed. The principle of CHAOR was studied by non-denaturing electrophoresis. To detect the amplification product, a polyperoxidase-streptavidin conjugate (molar ratio 1:80) and an enhanced chemiluminescence reaction were used, which made it possible to increase assay sensitivity.

An ultrasensitive bunge bedstraw herb type DNA machine for absolute quantification of mRNA in single cell.

Messenger ribonucleic acids (mRNAs) comprise a class of small nucleic acids carrying genetic information, which exhibit very important role in medical research and diagnosis. If only the mean mRNA expression levels of the mRNA population are considered in medical research, important information linking mRNA expression and cellular function may be lost. Single-cell analysis provides valuable insights into studying its heterogeneity, signaling, and stochastic gene expression.