[Biosynthesis of proteins in various organs of albino rats in experimental peritonitis].

Protein biosynthesis and the state of polysomes were studied in rat liver and spleen tissues under conditions of acute purulent peritonitis of abdominal cavity. In the both tissues studied synthesis of proteins was markedly inhibited at the initial period of the peritonitis development within 6 hrs, these patterns were further increased within 12-48 hrs but they remained lower the control values. The structure-functional impairments of polysomes were most distinct within 24-48 hrs after the disease beginning.

[Microbiological determination of DNA levels in blood leukocytes].

A procedure is developed for estimation of DNA microquantities in biological samples, which involved acid hydrolysis of DNA and the following microbiological estimation of its concentration. A sensitivity of the procedure was as little as 0.25 micrograms DNA per ml.

[Biosynthesis of nucleic acids and proteins in different time periods of development in experimental myocarditis].

Biosynthesis of DNA, RNA and protein was studied in rat myocardium within 1, 3, 5, 7, 14, 21 and 28 days after administration of adrenaline toxic doses (300 mg/100 g of body mass). Synthesis of DNA was activated within 1-7 days, of total proteins--within 1-14 days, of RNA--within 1-"1 days, of action and myosin in myocardium--within 3-21 days. All the patterns studied were normalized within the subsequent periods (21-28 days) although they exceeded the control values.

[RNA biosynthesis in various organs of the rat in experimental peritonitis].

Synthesis of various RNA species was studied in liver, spleen, and kidney tissues of one year old Wistar rat males within 6, 12, 24 and 48 hrs after peritonitis development. RNA biosynthesis was found to be unaltered in kidney; in liver tissue it was distinctly decreased beginning from 24 hrs and lowered down to 40% of the control value within 2 days. In spleen an increase in synthesis of pro-mRNA (90%) and mRNA was observed at early steps of peritonitis, with the subsequent decrease of these patterns within 24-48 hrs as compared with the previous periods of the disease as well as with controls.

[Cytogenetic effect of barbiturate anesthetizing substances in a human peripheral blood leukocyte culture].

It is found that hexenal and sodium thiopental in vitro produced a two-fold increase of frequency of chromosome aberrations as compared with the control and this effect was not dose-dependent. The anesthetics under study affected in vitro 3H-thymidine incorporation into DNA of lymphocytes, and a ten-fold hexenal dose intensified 3H-thymidine incorporation. The frequency of chromosome aberrations in vivo was at the level of the spontaneous mutation after use of sodium thiopental and slightly increased hexenal.