Effect of buffer additives on solubilization and refolding of reteplase inclusion bodies.

Reteplase is a non-glycosylated and recombinant form of tissue type plasminogen activator, which is produced in . However, its overexpression usually leads to formation of inactive aggregates or inclusion bodies. In the present study, we report on the development of optimized processes for isolation, solubilization, and refolding of reteplase inclusion bodies to recover active protein.

An Automatic Prolongation Detection Approach in Continuous Speech With Robustness Against Speaking Rate Variations.

In recent years, many methods have been introduced for supporting the diagnosis of stuttering for automatic detection of prolongation in the speech of people who stutter. However, less attention has been paid to treatment processes in which clients learn to speak more slowly. The aim of this study was to develop a method to help speech-language pathologists (SLPs) during diagnosis and treatment sessions.

Gene Diversity of Trichomonas vaginalis Isolates.

Background: Trichomonas vaginalis is protozoan parasite responsible for trichomoniasis and is more common in high-risk behavior group such as prostitute individuals. Interest in trichomoniasis is due to increase one's susceptibility to viruses such as herpes, human papillomavirus and HIV. The aim of this study was to find genotypic differences between the isolates.