[Features of ozone effect on the oxygen-dependent blood processes under hypercapnia conditions].

The aim of this work is to study of ozone effect on blood oxygen-dependent processes under hypercapnia conditions. The studied blood samples are pretreated with a hypercapnic gas mixture followed by the addition of ozonized isotonic sodium chloride solution (with an ozone concentration of 6 mg/l), as well as gaseous transmitters donors, nitroglycerin and sodium hydrosulfide. It has been established that hypercapnia enhanced the ozone effect on the blood oxygen transport function and was characterized by the oxyhemoglobin dissociation curve shift to the right, also increased hydrogen sulfide synthesis and absence of changes in the nitrates/nitrites concentration.

[Prooxidant-antioxidant balance depending on endothelial nitric oxide synthase G894T polymorphism].

The aim of the study was to assess the prooxidant-antioxidant balance depending on endothelial nitric oxide synthase G894T polymorphism. The frequency distribution of alleles and genotypes of G894T polymorphism, nitrite concentration, hydrogen sulphide, lipid peroxidation products (diene conjugates, malonic dialdehyde), antioxidants (reduced glutathione, catalase, ceruloplasmin, retinol) were determined in venous blood of healthy males. The incidence of the GG genotype was 49.

[THE INFLUENCE OF CARBON MONOXIDE ON THE PARAMETERS OF PROOXIDANT-ANTIOXIDANT BALANCE DURING HEPATIC ISCHEMIA-REPERFUSION SYNDROME IN RATS].

The influence of carbon monoxide on the blood and liver prooxidant-antioxidant parameters: lipid peroxidation products (conjugated dienes, malondialdehyde, Schiff bases) and antioxidant system parameters (catalase, retinol, α-tocopherol, glutathione) were investigated in rats during hepatic ischemia-reperfusion. Hepatic ischemia was induced by Pringle maneuver for 30 min, reperfusion period was 120 min. It is detected, that hepatic ischemia-reperfusion leads to intensification of lipid peroxidation with declining of antioxidant defense.

Thiamine triphosphate and thiamine triphosphatase activities: from bacteria to mammals.

In most organisms, the main form of thiamine is the coenzyme thiamine diphosphate. Thiamine triphosphate (ThTP) is also found in low amounts in most vertebrate tissues and can phosphorylate certain proteins. Here we show that ThTP exists not only in vertebrates but is present in bacteria, fungi, plants and invertebrates.