The Prevalence of High Carcinogenic Risk of HPV Genotypes among HIV-Positive and HIV-Negative MSM from Russia.

Objective: Men who have sex with men (MSM) have a high risk of lifelong anal cancer caused by high-risk human papillomavirus (HR HPV) infections. The aim of this study was to investigate the prevalence of anal canal HR HPV infection among men who have sex with men (MSM) with and without HIV infection in Moscow (Russia). We evaluated associations of some HIV coinfections (HSV and CMV) and HPV distribution among MSM with and without HIV infection.

[Detection of LY6G6D gene exon-intron structure,].

Earlier we have shown high frequency of loss of heterozygosity of microsatellite marker D6S273 within HLA III class region in DNA samples from cervical intraepithelial neoplasias and cervical cancer. According to publications three genes were identified in this region. For detection of D6S273 position we used in silico analysis of mRNA sequences deposited in GenBank (NCBI) and investigated LY6G6D gene expression in tumor cell lines.

[c-Kit and PDGFRA mutations and clinico-morphological features of gastrointestinal stromal tumors].

Gastrointestinal stromal tumors (GIST) are the most common mesenchymal neoplasms of the digestive tract. Inherent overexpression of receptor tyrosine kinase KIT (CD117) and mutations in c-Kit or PDGFRA genes are highly significant prognosticators. A first Russian investigation of c-Kit and PDGFRA mutations in GIST was carried out in 60 patients.

[Morphological analysis of genetically damaged thyrocytes in nodular pathology of the thyroid gland].

The smears of isolated thyrocytes with micronuclei taken from 25 patients with nodular pathology of the thyroid gland were investigated. In all kinds of benign nodular pathology the number of micronucleated thyrocytes comes to 0.1-0.

[Isolation of cells for cytological and cytogenetic studies of the thyroid epithelium].

This paper presents a detailed description of the method for obtaining of high quality smears of thyrocytes from the fragments of rat (30 animals) and human (20 cases) thyroid gland using the method of enzymatic dissociation in 0.25% aqueous solution of collagenase (collagenase for pancreatic islet isolation type XI, collagen digestive activity 1205 units/mg, Sigma, USA) at 37 degrees C during 1; 1.5; 2; 2.