Publications by authors named "I Demyanchuk"

Programmed cell death (PCD) is relevant to many aspects in the growth and development of a plant organism. In their reproduction, many flowering plant species possess self-incompatibility (SI), that is an intraspecific reproductive barrier, which is a genetic mechanism ensuring the avoidance of inbreeding depression by preventing self-pollination. This phenomenon enhances intraspecific variation; however, SI is rather a hindrance for some fruit plant species (such as plum, cherry, and peer trees) rather than an advantage in farming.

View Article and Find Full Text PDF
Article Synopsis
  • Researchers are developing new strategies to improve the production and purification of recombinant proteins in plants by optimizing gene codon composition and exploring regulatory sequences.
  • A case study on interferon-αA demonstrated that using a thermostable protein stabilizer (lichenase) significantly enhances the yield of recombinant proteins without negatively affecting their biological activity.
  • The combination of protein stabilization and methods for purifying fusion proteins leads to efficient and cost-effective approaches for producing valuable proteins in plant systems.
View Article and Find Full Text PDF

We studied ternary mixtures of nonionic surfactant (C12E6, n-dodecyl hexaoxyethylene glycol monoether), polymer (PEG, polyethylene glycol), and water. A small amount of PEG induces demixing into the polymer-rich and surfactant-rich phases in the ternary PEG/C12E6/water mixture. Above a certain concentration and/or molecular weight of PEG, the surfactant-rich phase orders, even in a solution consisting of a few percent of surfactant.

View Article and Find Full Text PDF

We studied the separation process in the ternary mixtures of nonionic surfactant (C(12)E(6), hexaethylene glycol monododecyl ether), polymer (PEG = poly(ethylene glycol)), and water. The separation process of PEG/water rich domains from the surfactant rich matrix was observed by the optical microscopy. From the morphological analysis, we determined the size of the domains as a function of time.

View Article and Find Full Text PDF

A small-angle light scattering (SALS) technique together with optical microscopy observation are used to investigate phase separation kinetics in films of low molecular weight thermotropic liquid crystal (4-cyano-4'-n-octyl-biphenyl, 8CB) with flexible polymer (polystyrene, PS). The growth of domains is studied as a function of time, film thickness, and film composition. The light scattering results are correlated with the images obtained by optical microscopy observation.

View Article and Find Full Text PDF