Micronuclei in lymphocyte subsets in relation to immune proteins and allergic disease.

This is an investigation of 54 boys and 23 girls with a median age of 19 years (range 18-22 years). The study group contained 12 boys and five girls with asthma and 23 boys and seven girls with allergic rhinitis. Sensitivity to pollen and furred animals were reported by 22 boys and eight girls and by 17 boys and six girls, respectively.

Increased frequencies of micronuclei in T8 lymphocytes of smokers.

Micronucleus frequencies and mitotic indices were analyzed in B, T4, and T8 lymphocytes from 40 smokers and 42 non-smoking referents. The highest level of micronuclei was found in T4 cells followed by T8 and B cells. These differences were statistically significant.

Micronuclei and mitotic index in B-, T4- and T8-cells treated with mitomycin C and gamma-irradiation.

Lymphocytes were treated in vitro with mitomycin C and gamma-radiation at different doses (0-250 nmol/l and 0-2 Gy, respectively). After incubation in RPMI 1640 medium and stimulation with phytohemagglutinin for 72 h, the lymphocyte subgroups T4 (CD4), T8 (CD8) and B (CD19) were separated by an immunomagnetic method and analyzed for the presence of micronuclei. With mitomycin C the highest levels were found in T4- and B-cells.

Effect of acid precipitation on retention and excretion of elements in man.

From a population of 8918 farmers, 237 were selected whose consumption of locally produced foods was high. The subjects' water sources, private wells, were of different degrees of acidity. Significant associations between pH (median 6.