Inhibition of the IL-15 pathway in anti-CD25 mAb treated renal allograft recipients.

Anti-CD25 mAb's are used for prophylaxis of rejection in allograft transplantation. These agents target the alpha-chain, part of the IL-2Ralphabetagamma complex. The beta- and gamma-chain are signaling components that are not specific for IL-2.

Eicosanoid production by the mucosa in inflammatory bowel disease after 5-ASA treatment.

Eicosanoids were measured in biopsies of colonic mucosa from five patients with active ulcerative colitis before and after a four weeks of treatment with 5-amino-salicylic acid (5-ASA). Eicosanoid formation was determined after the addition of 14C-arachidonic acid and stimulation with calcium ionophore A23187. 5-ASA given intra-rectally caused a non-selective suppression of prostanoids, leukotrienes and hydroxy-eicosatetraenoic acids.

15-HETE is the main eicosanoid formed by human colonic mucosa.

Eicosanoids were measured in colonic biopsies from eleven patients with active ulcerative colitis and from thirteen controls. Eicosanoid formation was measured after the addition of arachidonic acid and stimulation with calcium ionophore A23187. The 15-lipoxygenase derivative 15-hydroxy-eicosatetraenoic acid (15-HETE) was the predominant product formed in all biopsies.