Determination of binding affinity of enantiomers to albumin by liquid chromatography.

The principles of the determination of the binding affinity constants of small molecules to albumin by liquid chromatography, using albumin as a mobile phase additive, are outlined. Chromatographic conditions for determinations of constants are presented and applied to enantiomers of tryptophan and omeprazole. The influence of albumin on the retaining properties of LiChrosorb RP-8, Phenyl Hypersil and LiChrosorb Diol was studied.

Chromatographic resolution of enantiomers using albumin as complexing agent in the mobile phase.

Enantiomers of carboxylic acids have been separated with albumin as a chiral complexing agent in the mobile phase. Stereoselective separation has been obtained for different types of acids, in some cases with very high separation factor, as shown for di-p-toluoyltartaric acid (alpha(s) = 5.8).

Solvent extraction of tricyclic amines from blood plasma and liquid chromatographic determination.

The extraction of seven tricyclic antidepressant amines from human plasma at different pH values was investigated for dichloromethane, diethyl ether and hexane--1-pentanol (95:5). The amines were extracted as bases and back-extracted to sulphuric acid, 0.10 mol/l, prior to the separation by bonded-phase liquid chromatography.