A Disposable Pipette Extraction-UHPLC-MS/MS Method Based on Removal of Phospholipids to Determine Anandamide, 2-Arachidonoylglycerol, Cannabidiol, and Δ-Tetrahydrocannabidiol in Plasma Samples.

Cannabidiol (CBD) and Δ-tetrahydrocannabinol (THC), the main components of Cannabis sativa plants, can interact with specific cell receptors known as cannabinoid receptors (CBs). The endogenous compounds anandamide (AEA) and 2-arachidonoylglycerol (2-AG) are CB agonists, and, alongside enzymes, they constitute the endocannabinoid system (ECS) and take part in neuromodulation. Several LC-MS/MS methods have been developed to quantify these compounds in biological matrixes, but a fast and simple method that can determine these analytes in plasma samples simultaneously is not available.

Chronic fat consumption affects metabolic aspects of murine physiology and influences ovarian follicle atresia.

Saturated fat has been linked to cardiovascular diseases, leading to an increase in polyunsaturated fat consumption. The aim of the present study was to investigate the effects of three fat sources (coconut oil, lard and soybean oil) on metabolic and reproductive parameters in heterogenic mice. Female Swiss mice (5-6 weeks old; n=9/group) were divided into four experimental groups: control (CC), coconut oil (CO), lard (LA), and soybean oil (SO), and were orally given 0.

Periventricular Nodular Heterotopias Induced-Seizures in an Adolescent.

A female adolescent with no relevant past history was admitted to the Pediatric Emergency Department with two episodes of seizures without trauma, fever, or other symptoms. Head-MRI revealed bilateral subependymal nodular irregularities lining the lateral ventricles, with similar signal evolution to grey matter, confirming the diagnosis of periventricular nodular heterotopias (PVNH). Genetic testing revealed a Filamin A ( variant; family studies were negative.

Soil Application of Selenium in Wheat ( L.) Under Water Stress Improves Grain Quality and Reduces Production Losses.

Selenium (Se) is an essential element for humans. However, much of the world's human population is deficient in this element, which has become a public health problem. This study aimed to evaluate whether applying severe water stress to wheat plants ( L.

GLYCINE betaine and seaweed-based biostimulants improved leaf water status and enhanced photosynthetic activity in sweet cherry trees.

Sweet cherry is a high-value crop, and strategies to enhance production and sustainability are at the forefront of research linked to this crop. The improvement of plant status is key to achieving optimum yield. Biostimulants, such as glycine betaine (GB) or seaweed-based biostimulants [e.