Publications by authors named "I Borodina"
To cover the rising demand for natural food dyes, new sources and production methods are needed. Microbial fermentation of nature-identical colours, such as the red pigment betanin, has the potential to be a cost-efficient alternative to plant extraction. The last step of betanin production is catalysed by a UDP-glycosyltransferase (UGT).
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- Digital microfluidics technology can improve biological experiments but faces issues like biofouling that slow processes and cause contamination.
- Traditional solutions like surfactants reduce fouling but can disrupt biological reactions, reducing efficiency.
- This study shows that slippery liquid-infused porous surfaces (SLIPS) effectively prevent biofouling and enhance PCR performance in DMF devices, leading to faster experiments and reduced reagent usage without compromising reaction integrity.
Combinatorial library-based metabolic engineering approaches allow lower cost and faster strain development. We developed a genetic toolbox EXPRESS for combinatorial engineering of the oleaginous yeast Yarrowia lipolytica. The toolbox enables consecutive rounds of engineering, where up to three combinatorially assembled gene expression cassettes can be integrated into each yeast clone per round.
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- Clozapine can be toxic when it builds up in white blood cells, so understanding the transporters involved is important.
- Researchers developed a CRISPR-Cas9 yeast library to identify which transporters were responsible for clozapine resistance, discovering that the absence of the MDL1 transporter led to increased resistance.
- The study indicates that the mitochondrial MDL1 transporter is crucial for clozapine accumulation, with its human equivalent being ABCB10.
An experimental study of the dependence of the electrical impedance of a lateral electric field resonator on its thickness and the size of the gap between the electrodes was carried out. The resonator was made of PZT-19 piezoceramics in the form of a rectangular parallelepiped with the shear dimensions of 18 × 20 mm. Two rectangular electrodes with a gap that varied in the range from 4 to 14 mm were applied on one side of the resonator.
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