Localization of Plum pox virus in stem and petiole tissues of apricot cultivars by immuno-tissue printing.

Localization of Plum pox virus (PPV) in stem and petiole tissues of four susceptible and four resistant apricot cultivars has been studied. Consecutive 1-mm spaced transverse sections were taken from the tissues and were printed onto nitrocellulose membrane in duplicate. For virus-specific detection, one series of prints was probed with an antibody to PPV coat and the density of stains was evaluated by light microscopy.