Publications by authors named "I Bekeredjian-Ding"
Gut microbiota-derived metabolites play a pivotal role in the maintenance of intestinal immune homeostasis. Here, we demonstrate that the human commensal possesses a specific metabolic fingerprint, consisting predominantly of the tryptophan catabolite indole-3-propionic acid (IPA), the branched-chain acids (BCFAs) isobutyrate and isovalerate and the short-chain fatty acids (SCFAs) acetate and propionate. Mono-colonization of germ-free mice with (CS mice) affected colonic mucosal immune cell phenotypes, including up-regulation of gene expression, and increased abundance of transcriptionally active colonic tuft cells and Foxp3 regulatory T cells (Tregs).
View Article and Find Full Text PDFThe host-microbiome axis has been implicated in promoting anti-inflammatory immune responses. Yet, the underlying molecular mechanisms of commensal-mediated IL-10 production by regulatory B cells (Bregs) are not fully elucidated. Here, we demonstrate that bacterial CpG motifs trigger the signaling downstream of TLR9 promoting IκB-mediated expression of Blimp-1, a transcription regulator of IL-10.
View Article and Find Full Text PDFArticle Synopsis
- The study explores a new method for testing the potency of diphtheria, tetanus, and acellular pertussis (DTaP) vaccines, shifting from traditional in vivo animal testing to simultaneous serological testing on guinea pigs.
- It used established WHO International Standards as references and developed a quantification technique for detecting IgG levels against the vaccine antigens through an advanced immunoassay.
- The results demonstrated the effectiveness of this serological approach in identifying vaccine potency and safety, paving the way for its routine application in place of in vivo animal tests, aligning with ethical considerations pertinent to the 3Rs principle (Replace, Reduce, Refine).
Article Synopsis
- The study focuses on improving blood safety measures by validating Transfusion-Relevant Bacterial Reference Strains (TRBRS) in a lyophilised format, making them easier to use compared to current methods requiring ultra-cold storage.
- Two bacterial strains, Klebsiella pneumoniae and Staphylococcus aureus, were lyophilised and sent to 11 labs globally to test their effectiveness in identifying and monitoring growth in platelet concentrates.
- Results showed that the lyophilised TRBRS maintained growth properties and stability, allowing for straightforward addition to platelet concentrates, and demonstrated effectiveness across all participating labs, indicating potential for broader application in blood safety quality control.