Publications by authors named "I Bantounas"
Article Synopsis
- The study investigates HNF1B, a gene that encodes a transcription factor important for kidney development, and its mutations that lead to kidney malformations.
- Researchers created kidney organoids with HNF1B mutations using CRISPR-Cas9 technology to study the resulting abnormalities at a cellular level.
- Findings revealed that these mutant organoids had deformed tubules and altered gene expression affecting key pathways, contributing to a better understanding of how HNF1B mutations cause kidney disease.
Article Synopsis
- The Golgi complex is a network of stacked membranes near the cell nucleus, and its shape and position depend on interactions with the cell's microtubules and actin.
- This study reveals that the Golgi is closely associated with vimentin intermediate filaments in mouse and human cells, and that the protein GORAB interacts with these filaments.
- The absence of vimentin and/or GORAB affects the stability and integrity of the Golgi, leading to quicker disassembly and slower reassembly after disruptions, indicating their role in supporting the Golgi complex.
MicroRNAs are small RNAs that negatively regulate gene expression and play an important role in fine-tuning molecular pathways during development. There is increasing interest in studying their function in the kidney, but the majority of studies to date use kidney cell lines and assess the total amounts of miRNAs of interest either by qPCR or by high-throughput methods such as next generation sequencing. However, this provides little information as to the distribution of the miRNAs in the developing kidney, which is crucial in deciphering their role, especially as there are multiple kidney cell types, each with its own specific transcriptome.
View Article and Find Full Text PDFMicroRNAs (miRNAs) are gene expression regulators and they have been implicated in acquired kidney diseases and in renal development, mostly through animal studies. We hypothesized that the miR-199a/214 cluster regulates human kidney development. We detected its expression in human embryonic kidneys by in situ hybridization.
View Article and Find Full Text PDFBackground: Numerous studies have documented the in vitro differentiation of human pluripotent stem cells (hPSCs) into kidney cells. Fewer studies have followed the fates of such kidney precursor cells (KPCs) inside animals, a more life-like setting. Here, we tested the hypothesis that implanting hPSC-derived KPCs into an in vivo milieu surgically engineered to be highly vascular would enhance their maturation into kidney tissues.
View Article and Find Full Text PDF