Publications by authors named "I B Sidorov"
Article Synopsis
- Rhabdomyosarcomas (RMS), particularly the alveolar subgroup, are prevalent in children and adolescents, with significant genetic features including common translocations of specific genes.
- The study analyzed 32 tumor samples using various methods, revealing 81% had rearrangements at the FOXO1 locus, and identified translocation partners in most samples.
- The findings highlighted genetic diversity in alveolar RMS, with four distinct cytogenetic patterns observed, and noted that approximately 20% of cases showed no signs of genetic rearrangement.
Introduction: BRAF V600E substitution predicts sensitivity of a cancer to BRAF inhibitor therapy. The mutation is rarely found in soft-tissue sarcomas. Here we describe a case of undifferentiated spindle cell sarcoma showing primary insensitivity to standard chemotherapy and pronounced but non-sustained response to BRAF/MEK inhibitors at recurrence.
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- Metagenomics helps diagnose infections, but there hasn't been much comparison of methods for finding viruses across different labs.
- A study was done by the European Society for Clinical Virology to test twelve different lab methods using a special reference panel that simulates low amounts of viruses.
- The results showed that most methods could find common viruses, but some struggled with very low amounts, suggesting labs need to follow the same standards for better results.
Unlabelled: Viral enrichment by probe hybridization has been reported to significantly increase the sensitivity of viral metagenomics. This study compares the analytical performance of two targeted metagenomic virus capture probe-based methods: (i) SeqCap EZ HyperCap by Roche (ViroCap) and (ii) Twist Comprehensive Viral Research Panel workflow, for diagnostic use. Sensitivity, specificity, and limit of detection were analyzed using 25 synthetic viral sequences spiked in increasing proportions of human background DNA, eight clinical samples, and American Type Culture Collection (ATCC) Virome Virus Mix.
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- Various cell culture models have been used for SARS-CoV-2 research, including Vero and Calu-3 cells, but they each have their drawbacks.
- Human ACE2-expressing H1299 cells offer a more efficient alternative, as they are easy to manipulate and support high levels of viral replication while displaying a functional immune response.
- These H1299/ACE2 cells are particularly useful for conducting antiviral assays and studying variants like omicron, making them a valuable tool in understanding the virus and developing treatments.