[Case of anterior ischemic optic neuropathy accompanied by Churg-Strauss syndrome].

Background: Churg-Strauss syndrome (CSS) is a vasculitis which includes the three signs of bronchial asthma, hypereosinophilia, and vasculitis syndrome. We report a case in whom anterior ischemic optic neuropathy (AION) accompanied CSS. Such a case has not been reported previously in Japan.

lambda-crystallin related to dehydroascorbate reductase in the rabbit lens.

Purpose: To evaluate the relationship of lambda-crystallin to reduced nicotinamide adenine dinucleotide (NADH)-dependent dehydroascorbate (DHA) reductase found specifically in the rabbit lens.

Methods: DHA reductase Fractions I-IV were separated from the lambda/betaL1-crystallin fraction of rabbit lens soluble protein by diethylaminoethyl (DEAE)-cellulose ion-exchange column chromatography, and then the enzyme was partially purified from Fraction II by rechromatography on the same ion-exchange column. The isolated DHA reductase fractions were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting, native isoelectric focusing and two-dimensional gel electrophoresis.

NADH-dependent dehydroascorbate reductase in the rabbit lens.

The present investigation demonstrates the existence of NADH-dependent dehydroascorbate (DHA) reductase activity in the soluble fraction of the rabbit lens. This DHA reductase was specific for NADH, and its apparent Km values for DHA and NADH were 5.7 mM and 4.

Simultaneous determination of GTP cyclohydrolase activity and biopterin content in pheochromocytoma PC12h cells.

A method to measure the GTP cyclohydrolase and biopterin (BP) content at the same time in the sonicate of pheochromocytoma PC12h cells was described. The cells cultured in a 5-cm dish were sonicated with 0.4 to 0.